The opening of the confocal imaging facility is organized at Ruder Boskovic Institute in Zagreb in Februady 27, 2014. The meeting consists of lectures focused on the current advanced methods in confocal microscopy nowdays, and application lectures. In the afternoon, there will be introduced the best microscope worldwide for the advanced image acquisition, live cells imaging and spectral based FLIM – Leica TCS SP8 X SMD.

The confocal imaging facility is equipped with newly installed Leica TCS SP8 X SMD and Leica TCS SP2 AOBS confocal laser scanning microscopes. Leica TCS SP8 X SMD is equipped with:
– pulsed White Light Laser with full tunability in 1 nm step size in the spectrum 470-670 nm
– tandem scanner consisting of FOV scanner with maximum file format 64 MPx, and the fastest scanner worldwide with 40 fps at 512×512 pxls in several spectral detectors
– combination of PMT, FLIM-PMT and HyD spectral detectors for and ideal optimalization of experimental setup
– PicoQuant detection for two channel spectral detection of FLIM

The Advanced Techniques Day focused on advanced microscopy technique of superresolution microscopy STED and novel 3D STED will be held at Vinicna 7, Prague 2 (lecture hall B3-Fotochemie). This technique is able to scan your samples in a very low illumination volume down to 50 nm in xy plane and <120 nm in z plane without additional data processing.

12. 12. Superresolution microscopy (STED & 3D STED) plus confocal bits

9:00 – 10:00 Rolf Borlinghaus (Senior Scientist, Leica Microsystems), Three Dimensions in Microscopy – spectral confocal and a preview on 3D-STED
10:00 – 11:00 Alberto Diaspro (Director Nanophysics IIT Genova), Pushing STED further
11:00 – 11:15 Coffee break
11:15 – 12:00 Bettina Griesshaber (Product Manager Confocal DSA, Leica Microsystems), Deeper imaging with TCS SP8 MP
12:00 – 13:00 Summary and discussion